Standardisation and establishment of a rabies ELISA test in European laboratories for assessing the efficacy of oral fox vaccination campaigns.
Identifieur interne : 000216 ( France/Analysis ); précédent : 000215; suivant : 000217Standardisation and establishment of a rabies ELISA test in European laboratories for assessing the efficacy of oral fox vaccination campaigns.
Auteurs : Florence Cliquet [France] ; Thomas Müller ; Franco Mutinelli ; Sabrina Geronutti ; Bernard Brochier ; Thomas Selhorst ; Jean-Luc Schereffer ; Nicolas Krafft ; Jeanette Burow ; Astrid Schameitat ; Hartmut Schlüter ; Michel AubertSource :
- Vaccine [ 0264-410X ] ; 2003.
English descriptors
- KwdEn :
- Administration, Oral, Animals, Antibodies, Viral (blood), Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (standards), Europe, Fluorescent Antibody Technique, Foxes (immunology), Laboratories (standards), Neutralization Tests, Rabies (immunology), Rabies (prevention & control), Rabies (veterinary), Rabies Vaccines (administration & dosage), Rabies Vaccines (immunology), Reference Standards, Sensitivity and Specificity, Vaccination (veterinary).
- MESH :
- chemical , administration & dosage : Rabies Vaccines.
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Rabies Vaccines.
- geographic : Europe.
- immunology : Foxes, Rabies.
- methods : Enzyme-Linked Immunosorbent Assay.
- prevention & control : Rabies.
- standards : Enzyme-Linked Immunosorbent Assay, Laboratories.
- veterinary : Rabies, Vaccination.
- Administration, Oral, Animals, Fluorescent Antibody Technique, Neutralization Tests, Reference Standards, Sensitivity and Specificity.
Abstract
A simple and rapid enzyme linked immunosorbent assay (ELISA) to detect rabies antibodies in field fox sera has been standardised and established in several European laboratories. The same panels of 100 coded sera were investigated by four laboratories using this ELISA assay and reference serum neutralisation techniques (fluorescent antibody virus neutralisation (FAVN) test and rapid fluorescent focus inhibition test (RFFIT)). This indirect ELISA technique is highly correlated with conventional seroneutralisation test on cell culture. Results obtained with this ELISA test provide an almost perfect agreement between laboratories while the agreement of FAVN test and RFFIT results is substantial and very satisfactory. In view of this study, this simple and rapid ELISA test is a suitable tool for evaluating the sero-conversion rate in fox populations following oral rabies vaccination campaigns in European countries.
PubMed: 12798642
Affiliations:
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pubmed:12798642Le document en format XML
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The same panels of 100 coded sera were investigated by four laboratories using this ELISA assay and reference serum neutralisation techniques (fluorescent antibody virus neutralisation (FAVN) test and rapid fluorescent focus inhibition test (RFFIT)). This indirect ELISA technique is highly correlated with conventional seroneutralisation test on cell culture. Results obtained with this ELISA test provide an almost perfect agreement between laboratories while the agreement of FAVN test and RFFIT results is substantial and very satisfactory. In view of this study, this simple and rapid ELISA test is a suitable tool for evaluating the sero-conversion rate in fox populations following oral rabies vaccination campaigns in European countries.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Malzeville</li></settlement></list><tree><noCountry><name sortKey="Aubert, Michel" sort="Aubert, Michel" uniqKey="Aubert M" first="Michel" last="Aubert">Michel Aubert</name><name sortKey="Brochier, Bernard" sort="Brochier, Bernard" uniqKey="Brochier B" first="Bernard" last="Brochier">Bernard Brochier</name><name sortKey="Burow, Jeanette" sort="Burow, Jeanette" uniqKey="Burow J" first="Jeanette" last="Burow">Jeanette Burow</name><name sortKey="Geronutti, Sabrina" sort="Geronutti, Sabrina" uniqKey="Geronutti S" first="Sabrina" last="Geronutti">Sabrina Geronutti</name><name sortKey="Krafft, Nicolas" sort="Krafft, Nicolas" uniqKey="Krafft N" first="Nicolas" last="Krafft">Nicolas Krafft</name><name sortKey="Muller, Thomas" sort="Muller, Thomas" uniqKey="Muller T" first="Thomas" last="Müller">Thomas Müller</name><name sortKey="Mutinelli, Franco" sort="Mutinelli, Franco" uniqKey="Mutinelli F" first="Franco" last="Mutinelli">Franco Mutinelli</name><name sortKey="Schameitat, Astrid" sort="Schameitat, Astrid" uniqKey="Schameitat A" first="Astrid" last="Schameitat">Astrid Schameitat</name><name sortKey="Schereffer, Jean Luc" sort="Schereffer, Jean Luc" uniqKey="Schereffer J" first="Jean-Luc" last="Schereffer">Jean-Luc Schereffer</name><name sortKey="Schluter, Hartmut" sort="Schluter, Hartmut" uniqKey="Schluter H" first="Hartmut" last="Schlüter">Hartmut Schlüter</name><name sortKey="Selhorst, Thomas" sort="Selhorst, Thomas" uniqKey="Selhorst T" first="Thomas" last="Selhorst">Thomas Selhorst</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Cliquet, Florence" sort="Cliquet, Florence" uniqKey="Cliquet F" first="Florence" last="Cliquet">Florence Cliquet</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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